Jun 15, 2017 — who.int/csr/resources/publications/biosafety/Biosafety7.pdf. Appendix D Safety. Biological hazard safety. D. QuantStudio™ 5 Real-Time
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For Research, Forensic, or Paternity Use Only. For licensing and limited use restrictions visit thermo®sher.com/HIDlicensing. QuantStudio 5 Real-Time PCR Instrument(for Human Identi®cation)USER GUIDEInstallation, maintenance, and administration for use with: HID RealTime PCR Analysis Software v1.3 Publication Number MAN0017162Revision A.0

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Manufacturer: Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256 The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT. Revision history : Pub. No. MAN0017162Revision Date DescriptionA.015 June 2017New document for Human Identi®cation work¯ows using instrument ®rmware v1.3.1.Important Licensing Information : This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all applicable Limited Use Label Licenses. Trademarks : All trademarks are the property of Thermo Fisher Scienti®c and its subsidiaries unless otherwise speci®ed. Microsoft and Windows are trademarks of Microsoft Corporation. Pentium is a trademark of Intel Corporation. •2017 Thermo Fisher Scienti®c Inc. All rights reserved.

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Contents CHAPTER 1 Product information 7 Instrument overview .7 Installation, veri®cation, and calibration .8 Instrument ®lters and supported dyes 9 System dyes 9 Custom dyes ..10Parts of the instrument 10Parts of the home screen .11Comparison of features in desktop software and the instrument 13CHAPTER 2 Install the instrument 14Work¯ow: Install the instrument .14Before you begin installation .14Unpack and install the instrument ..15Power on and follow the startup wizard .15Instrument and computer connections ..16Computer-to-instrument con®guration.17Firewall ports that must be open ..17CHAPTER 3 General procedures to use the instrument .18Precautions for use 18Power on the instrument ..19Power off the instrument .19Sign in20Sign out..20Load and unload plate in the instrument 21View real-time data and plots on the instrument touchscreen 22Adjust the display of real-time plots on the instrument touchscreen ..22Transfer EDS ®les from the instrument home screen .22QuantStudio 5 Real-Time PCR Instrument User Guide (for Human Identi®cation) | MAN0017162 A.03

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CHAPTER 4 Con®gure the instrument and manage instrument pro®les.23Initial start up ..23Overview of instrument settings (Administrator) ..24Overview of instrument settings (Standard or Guest) .26Manage instrument pro®les..27Instrument pro®les and signed-in users ..27Create an administrator instrument pro®le during initial start-up .27Create a new instrument pro®le28Edit an instrument pro®le28Manage all instrument pro®les.28Require instrument pro®le sign-in..29Manage the sign out timer29Manage the instrument name.29Enable sleep mode .30Set the idling temperature for the heated cover 30Set the date and time ..30Restore factory defaults ..31Enable Remote Monitoring Service ..31Update instrument software ..32CHAPTER 5 Calibrate and verify instrument performance 33Calibration and veri®cation schedule 34Calibration descriptions..35View calibration status and set reminders in the instrument .36Perform ROI/uniformity, background, and dye calibrations ..36Work¯ow: Calibration 36Prepare a calibration plate ..37Perform calibrations .38View calibration images .39Identify contamination 40Create a background plate (optional)..40Contents 4QuantStudio 5 Real-Time PCR Instrument User Guide (for Human Identi®cation) | MAN0017162 A.0

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Calibrate dyes for HID validated work¯ows.41Work¯ow: Calibrate dyes for HID validated work¯ows..41Prepare a calibration plate for HID validated work¯ows41Add custom dyes to the instrument for HID validated work¯ows..42Perform a custom dye calibration for HID validated work¯ows42Perform instrument veri®cation using RNase P plates 44Instrument veri®cation description44RNase P instrument veri®cation plate .45Performance speci®cations pass criteria .45Prepare an RNase P plate 46Perform RNase P veri®cation..47CHAPTER 6 Maintain the instrument 48Backup or restore the instrument 48Decontaminate the sample block .49Materials required 49Clean the sample block ..49Detailed procedures for cleaning the sample block ..51Replace the instrument fuses .52Materials required 52Replace the fuses .52Store, move, or ship the instrument .53Prepare the instrument to store, move, or ship ..53Move the instrument .53Return the instrument for service ..54APPENDIX A Troubleshooting ..55Instrument troubleshooting ..55Troubleshoot calibration failure ..56Troubleshoot veri®cation failure .57APPENDIX B Parts and materials .58Kits, consumables, accessories, and reagents .58Consumables (96 well, 0.2 mL format) ..59 Accessories .60General-use materials and consumables ..60Contents QuantStudio 5 Real-Time PCR Instrument User Guide (for Human Identi®cation) | MAN0017162 A.05

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APPENDIX C Instrument speci®cation and layout .61Con®gured system dimensions ..62Instrument and computer connections ..63Instrument clearances .64Electrical requirements 64Environmental requirements .65APPENDIX D Safety ..66Symbols on this instrument ..67Conformity symbols ..68Safety alerts on this instrument ..69Location of safety labels on the instrument ..69Safety information for instruments not manufactured by Thermo Fisher Scienti®c..70Instrument safety ..70General 70Physical injury 71Electrical ..72Cleaning and decontamination ..72Safety and electromagnetic compatibility (EMC) standards ..72Safety compliance 73EMC.73Environmental design 74Chemical safety .74Biological hazard safety 75Documentation and support 76Related documentation 76Obtain information from the Help system ..76Customer and technical support .76Limited product warranty .76Index ..77Contents 6QuantStudio 5 Real-Time PCR Instrument User Guide (for Human Identi®cation) | MAN0017162 A.0

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Instrument ®lters and supported dyes Peak ®lterColor Filter wavelength (nm)[1]Factory-calibrated dyesExample custom dyes ExcitationEmission x1-m1Blue470 † 15520 † 15 FAM ,SYBR Green SYT09 x2-m2Green520 † 10558 † 12VIC JOE,HEX, TET [2]x3-m3Yellow550 † 10587 † 10 NED, TAMRA ,ABY [3]Cy3x4-m4Orange580 † 10623 † 14ROX , JUN [3]Texas Red x5-m5Red640 † 10682 † 14 MUSTANG PURPLE, Cy5LIZx6-m6Deep-Red662 † 10711 † 12None [4]Cy 5.5[1] The central wavelengths are the optimized wavelengths. [2] The HEX and TET dyes from Thermo Fisher Scientific fall within the emission wavelength range of the system, therefore they can be added and adapted for use on the instrument. [3] HID-validated workflows use versions of ABY and JUN dyes that are considered custom dyes. [4] This filter set currently does not support any dyes supplied by Thermo Fisher Scientific. 1×1-m1 ‡ FAM , SYBR Green 2×2-m2 ‡ VIC3x3-m3 ‡ ABY, NED, Cy3, TAMRA4x4-m4 ‡ JUN, ROX, Texas Red 5×5-m5 ‡ Cy5, MUSTANG PURPLESystem dyes Chapter 1 Product information Instrument ®lters and supported dyes 1QuantStudio 5 Real-Time PCR Instrument User Guide (for Human Identi®cation) | MAN0017162 A.09

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Note: Parts of the instrument 1Touchscreen … Controls the instrument. 2USB port … For connection to an external network drive or external data storage device. 3Instrument drawer … Contains sample plate. Note: Custom dyes Chapter 1 Product information Parts of the instrument 110QuantStudio 5 Real-Time PCR Instrument User Guide (for Human Identi®cation) | MAN0017162 A.0

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Parts of the home screen 1Avatar and Instrument name 2Eject instrument drawer icon 3Help system icon 4Status dial 5Instrument pro®le name;instrument block type 6Settings button7Access templates buttons (not applicable for HIDvalidated work¯ows)8Connectivity icons 9Sign In (or My Pro®le) button Table 1 Parts of the home screen Element of the home screen Function Avatar and Instrument nameSet by the administrator to uniquely identify the instrument. Eject instrument drawer iconTouch to open or close the instrument drawer. Help icon Touch to launch the touchscreen Help system to access step-by-step instructions.Status dial When the instrument is not in use … Displays Set up run.Note: Instrument runs must be set up and started from the desktop software. When the instrument is in use … Displays the sample block temperature, the elapsed run time, and the run status. Note: Swipe the dial to the left or touch to access real-time views of the run. Instrument pro®le usernameand block type Displays the username of a signed-in user and the instrument block type. Note: If no user is signed in, the instrument defaults to the Guest pro®le.Chapter 1 Product information Parts of the home screen 1QuantStudio 5 Real-Time PCR Instrument User Guide (for Human Identi®cation) | MAN0017162 A.011

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